085 Possible facilitating roles of alarmins in the TCR-induced activation of CRTH2+Th2 cells in the atopic dermatitis lesion

Type 2 cytokines, especially IL-4 and IL-13, play central roles in the pathogenesis of atopic dermatitis (AD). Immune cells acquired immunity Th2 cells, innate immune such as group lymphoid (ILC2s) basophils are major source type cytokines AD lesions. It is well known that alarmins including thymic stromal lymphopoietin (TSLP), IL-33, IL-25 essential activation cells. However, it not clear whether also To investigate involvement lesions, we collected CRTH2+Th2 skin biopsy samples from 11 patients. After expanding T vitro using IL-2 for 4 days, stimulated with a cocktail several (TSLP, IL-25, IL-1β, IL-18) and/or TCR stimulation by anti-CD3/CD28 24 hours. Then performed flow cytometric analysis cytokine production (IL-4, IL-13) CRTH2+ each group. We found alarmin alone did induce under showed tendency to increase percentage IL-13-producing (6.27%±5.94%) compared (3.68%±3.28%), although difference was statistically significant (p=0.057). These results suggest exert facilitating TCR-induced may contribute both